Have to given any understanding or bond ? If not than can submit resignation to your OIC. You have to serve number a days as per probation rules.

Penalty only if you have given any bond or undertaking. 

1) Resignations of Group A & B railway servants, other than those holding administrative posts, serving on railways may be accepted by the General Manager.

2) The resignation submitted by Group A probationers of different services undergoing probationary training in various Centralized Training Institutes may be accepted by the Heads of Centralized Training Institutes .

3) Resignation of all other Group A & B railway servants on Indian Railways/Production Units shall be forwarded to the Railway Ministry.

4) you can give  one month notice period And resign 

- As per Specific Relief Act, if any employee quits before the notice period, the Employer can only recover the Notice pay, and the Company cannot force to serve the entire notice period.

- Further, the resignation decision is the employee’s decision, and the employer cannot sue for breach of contract, if the employee leaves without serving contractual notice. 

- Probationary periods are often applied to new employees as a means of determining their capabilities in a new job.

- Further ,the employer can terminate during the probation , and employee can also leave the job with the terms and conditions mentioned in the appointment letter for probation. 

- Further , if an employee leave the job during the probation period , then it not harm his career, and generally many company stipulates in contract , that during probation period either party can terminate the job with or without any notices.

- Hence, legally no penalty can be imposed , if your resign with a valid reasons .

You can give your letter of resignation to your higher author after complying the service terms and conditions.

Please refer your appointment letter carefully and try to understand the terms mentioned therein. You would succeed to know your answer for resignation from your job during the probation period. 

You need to resign. Penalty if any will be stated in your contract or service agreement with them with clauses

Check if you have signed any bond. If yes you may have to fulfill its conditions. If no bond has been signed simply submit a resignation letter to General Manager of your railway

You would have been subjected to sign the terms and conditions while granting the EOL for study purpose.

You peruse the terms and conditions properly you will come to know what you are liable to pay to the government for resigning the job midway terminating the contract without any valid reason in the probation period.

This shall be as per contract you have with the IR so go through the contents of the contract letter you have (AL) and if there is some terms and conditions in this regard follow that rule.

1. You need to pay penalty as accepted by you at time of extraordinary leave sanctioning for study purpose.

2. You can resign from the post after payment of penalty charges.

Dear Sir,

You are suggested to confirm the details from the HR department regarding the rule for the resignation and penalty if there is any. You may also get the same information by way of RTI from the department and then you may act accordingly. 

EOL cannot be taken again. You want to resign then there muat be a bond period and if you leave then you have to pay the penalty. Refer to the contract.

write a resignation letter to your appointing authority. I’m accordance with the rules you will be required to serve a notice period. You may Peruse you’re service rules and interact with your office colleagues/HR to know more in this regard